Column: PrincetonSPHER C18, 100 Å, 5 μm
Catalog No: 150046-03501
Dimensions: 150 x 4.6 mm
Eluent: 60:40 A:B

A: 20mM NH2H2PO4 - pH 3.2
B: Acetonitrile

Flow Rate: 1.5 ml/min
Temperature: 40°C
Detection: UV at 280 nm
Injection: 10 μl (1 mg/ml sample)

1. Hydrochlorothiazide
2. Chlorthalidone
3. Diazoxide
4. Furosemide
5. Bendroflumethazide